Endogenous ADP-ribosylation during development of the prokaryote Myxococcus xanthus

Quantifying aggregation dynamics during Myxococcus xanthus development.

Under starvation conditions, a swarm of Myxococcus xanthus cells will undergo development, a multicellular process culminating in the formation of many aggregates called fruiting bodies, each of which contains up to 100,000 spores. The mechanics of symmetry breaking and the self-organization of cells into fruiting bodies is an active area of research. Here we use microcinematography and automat...

Inactivation of isocitrate lyase during myxospore development in Myxococcus xanthus.

The inactivation of isocitrate lyase which occurs during late stages of myxospore formation in Myxococcus xanthus was studied. Several findings are reported. (i) Protein synthesis is required over a specific time interval in order for isocitrate lyase inactivation to occur at a later time. (ii) Metabolic energy is required at all times during myxospore development if the inactivation is to occu...

Analyses of mrp genes during Myxococcus xanthus development.

Myxococcus xanthus is a gram-negative soil bacterium that undergoes development under starvation conditions. Our previous study identified a new genetic locus, mrp, which is required for both fruiting body formation and sporulation. The locus encodes two transcripts: mrpAB, which consists of a histidine kinase and an NtrC-like response regulator, and mrpC, a cyclic AMP receptor protein family t...

Changes in cyclic AMP levels during development in Myxococcus xanthus.

Cyclic AMP levels doubled in Myxococcus xanthus under conditions in which cells aggregate and form fruiting bodies. In liquid medium, glycerol- or dimethyl sulfoxide-induced sporulating cultures exhibited a sharp but transient rise in cyclic AMP concentration after 45 min.

CrdA regulates endogenous beta-lactamase activity in Myxococcus xanthus